Synthetic Chemically Modified mRNA
A “spike” sequence on a replication-competent plasmid with an SV40 promoter refers to a theoretical contamination scenario where the genetic material for a viral spike protein is inserted into a self-replicating plasmid DNA that can be activated by the potent SV40 promoter. This type of contamination has been a focus of concern and debate, particularly in the context of some COVID-19 vaccines
-> contamination includes replication-competent plasmid DNA encoding spike with an SV40 (cancer virus) promoter
BioNTech RNA-Based COVID-19 Injections Contain Large Amounts Of Residual DNA Including An SV40 Promoter/Enhancer Sequence
Abstract and Figures
“Background: BNT162b2 RNA-based COVID-19 injections are specified to transfect human cells to efficiently produce spike proteins for an immune response. Methods: We analyzed four German BNT162b2 lots applying HEK293 cell culture, immunohistochemistry, ELISA, PCR, and mass spectrometry. Results: We demonstrate successful transfection of nucleoside-modified mRNA (modRNA) biologicals into HEK293 cells and show robust levels of spike proteins over several days of cell culture. Secretion into cell supernatants occurred predominantly via extracellular vesicles enriched for exosome markers. We further analyzed RNA and DNA contents of these vials and identified large amounts of DNA after RNase A digestion in all lots with concentrations ranging from 32.7 ng to 43.4 ng per clinical dose. This far exceeds the maximal acceptable concentration of 10 ng per clinical dose that has been set by international regulatory authorities. Gene analyses with selected PCR primer pairs proved that residual DNA represents not only fragments of the DNA matrices coding for the spike gene, but of all genes from the plasmid including the SV40 promoter/enhancer and the antibiotic resistance gene. Conclusion: Our results raise grave concerns regarding the safety of the BNT162b2 vaccine and call for an immediate halt of all RNA biologicals unless these concerns can be dispelled.”
Conclusion
“We demonstrated that transfection of the human cellline HEK293 with four different BNT162b2 lotsresults in the production of spike proteins overseveral days, which are released into the cellsupernatant via exosomes. We detected residualplasmid-DNA in all vials at concentrations farexceeding the allowed EMA limit of 0.33 ng dsDNAper 1 mg RNA. We identified all plasmid genes aswell as the two copies of the 72 bp SV40promoter/enhancer element. The DNA was shown toenter and persist in the cells.Already before the start of the governmentalvaccination campaign, physicians and scientistspointed out that serious adverse events would betriggered by the gene-based agents. In themeantime, the spectrum of adverse side events has become so multifaceted that the term “spikeopathy”has been created to denote the new disease complex[53]. The eternal dangers of all RNA biologicals are4-fold: First, modRNA encoding any foreign proteinwill trigger detrimental autoimmune reactions [54].Second, the lipid nanoparticles are themselveshighly toxic [55]. Third, residual plasmid-DNA andreverse transcribed mRNA will genetically modifycells. Fourth, replacement of uridine in naturalmRNA by N1-methyl-pseudouridine in syntheticmodRNA causes +1 ribosomal frameshiftingresulting in haphazard production of utterly alienproteins [56].
Our results confirm and extend published reportsand raise grave concerns regarding the safety of theBNT162b2 vaccine. We call for an immediate halt ofall RNA-based biologicals until these concerns arescientifically addressed and convincingly dispelled.’
Source: Researchgate.net